Abstract The objective was to understand the difficulties and potential of a Mental Health (MH) network aimed at children and adolescents, identifying how professionals understand and operationalize the principles of the policy of SM for children and adolescents. Five Focus Groups were held, with the participation of 43 workers. We used lexical analysis through the Iramuteq software, originating four classes: “What is being a child and adolescent?”; “What do children and adolescents suffer from?”; “On the relationship between services” and “Potentials and challenges of the Mental Health Network”. Difficulties in sharing care were identified, articulating the network outside of oneself. Capsij is perceived as a priority for user embracement, and there is still difficulty in providing care in MH in Primary Care (AB). It is concluded that the complexity of care in MS for children and adolescents imposes the need to continue strategies to strengthen the AB and intersectoral actions aimed at an expanded clinic.

Resumen El objetivo fue comprender las dificultades y potencialidades de una red de Salud Mental (SM) para niños y adolescentes, identificando cómo los profesionales entienden y operacionalizan los principios de la política de SM para la niñez y adolescencia. Se realizaron cinco Grupos Focales, en los que participaron 43 trabajadores. Se utilizó el análisis léxico a través del software Iramuteq, originando cuatro clases: “¿Qué es ser niño y adolescente?”; “¿Qué padecen los niños y adolescentes?”; “Sobre la relación entre servicios” y “Potencialidades y desafíos de la Red de Salud Mental”. Se identificaron dificultades a la hora de compartir los cuidados, articulando la red para fuera de sí. El Capsij se percibe como una prioridad para la acogida, existiendo aún dificultades para implementar los cuidados en SM en Atención Primaria (AP). Se concluye que la complejidad de la atención en SM para niños y adolescentes impone la necesidad de continuidad de las estrategias de fortalecimiento de la AP y de acciones intersectoriales, visando una clínica ampliada.

Resumo Objetivou-se conhecer as dificuldades e potencialidades de uma rede de Saúde Mental (SM) voltada a crianças e adolescentes, identificando como profissionais compreendem e operacionalizam os princípios da política de SM infantojuvenil. Foram realizados cinco Grupos Focais, com participação de 43 trabalhadores. Utilizou-se da análise lexical por meio do software Iramuteq, originando quatro classes: “O que é ser criança e adolescente?”; “Do que sofrem as crianças e adolescentes?”; “Sobre a relação entre os serviços” e “Potencialidades e desafios da Rede de Saúde Mental”. Identificou-se dificuldades em compartilhar o cuidado, articulando a rede para fora de si. O Capsij é percebido como prioritário para o acolhimento, havendo, ainda, dificuldade em efetivar os cuidados em SM na Atenção Básica (AB). Conclui-se que a complexidade da atenção em SM para crianças e adolescentes impõe a necessidade de continuidade de estratégias de fortalecimento da AB e de ações intersetoriais visando uma clínica ampliada.

Abstract Purpose: This research aims to structure a hierarchical model that integrates the industry 4.0 (I4.0) concepts and standardizes concepts based on the literature. Originality/value: Kamble et al. (2018) point out the lack of architecture to represent I4.0 concepts. This paper brings an approach to the relationship between these concepts of I4.0. It expands the studies by Ghobakhloo (2018) and Liao et al. (2017) and homogenizes terms present in the literature. Design/methodology/approach: From a systematic review of the literature in the Scopus and ScienceDirect databases, from 2011 to 2019, 91 articles were reviewed, of which 58 articles were analyzed. Findings: From the literature, the terms related to I4.0 were grouped into three categories: technologies, principles, and dimensions. Technology clusters represent tools used to promote changes and transformations in the processes, here called principles. These changes and transformations create new industry standards, enabling process integration for problem-solving, and contributing to implementing intelligent management. The relationship between these categories results in a hierarchical model for I4.0 concepts. This hierarchical model can be used to identify opportunities for future research, demonstrating associations between categories that have not yet been explored. It opens possibilities for organizations to enter the fourth industrial revolution. The results help practitioners and researchers to understand this new process in detail and facilitate the construction of a valid and operational intelligent manufacturing platform.

Resumo Objetivo: Esta pesquisa tem como objetivo estruturar um modelo hierárquico que integre os conceitos da indústria 4.0 (I4.0) e padronizar conceitos com base na literatura. Originalidade/valor: Kamble et al. (2018) apontam para a falta de uma arquitetura para representar os conceitos I4.0. Este artigo traz uma abordagem para a relação entre esses conceitos de I4.0. Ele expande os es- tudos de Ghobakhloo (2018) e Liao et al. (2017) e homogeneíza termos presentes na literatura. Design/metodologia/abordagem: A partir de uma revisão sistemática da literatura nas bases de dados Scopus e ScienceDirect, de 2011 a 2019, foram revisados 91 artigos, dos quais 58 foram analisados. Resultados: A partir da literatura, os termos relacionados com I4.0 foram agrupados em três categorias: tecnologias, princípios e dimensões. Os clusters de tecnologia representam ferramentas utilizadas para promover mudanças e transformações nos processos, aqui chamados de princípios. Essas mudanças e transformações criam novos padrões da indústria, permitindo a integração de processos para a solução de problemas, contribuindo para a implementação do gerenciamento inteligente. A relação entre essas categorias resulta em um modelo hierárquico.

Abstract This article aims to know how the healthcare network has been operationalized from the perception of family members of children with demand for mental health care (MH). Two focus groups were held, one with family members from Primary Care (PC) and the other with family members from the Child Psychosocial Care Center (CAPSij), totaling 15 participants. A lexical analysis of the descending hierarchical classification type was performed with the help of the software R Interface for multidimensional analyzes of texts and questionnaires (IRAMUTEQ), resulting in five classes: The Magic Pill; Strengths and Weaknesses of services; Looking for help; Accepting the child’s diagnosis; and Where did I look for help. The results point to difficulties present in PC in identifying and managing situations of mental health in children and adolescents (MHCA), with a medicalization logic. Note that the school is presented as a prominent place in producing the demand for care, and the family is still not very much involved in the actions. It is, thus, concluded that advances are still needed for operationalization of care guided by MHCA policy guidelines.

Resumen Este artículo tuvo por objetivo conocer cómo opera una red asistencial a partir de la percepción de familiares de niños con demanda de atención en salud mental (SM). Se realizaron dos grupos focales, uno con familiares de Atención Primaria (AP) y otro con familiares del Centro de Atención Psicosocial Infantojuvenil (CAPSij), totalizando 15 participantes. Se realizó análisis léxico del tipo clasificación jerárquica descendente con la ayuda del software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires (IRAMUTEQ), lo que resultó en cinco clases: “La píldora mágica”; “Fortalezas y debilidades de los servicios”; “En busca de ayuda”; “Aceptar el diagnóstico del niño” y “¿Dónde busqué ayuda?”. Los resultados apuntan las dificultades presentes en AP para identificar y manejar situaciones de salud mental infantojuvenil (SMIJ) mediante una lógica aún medicalizante. La escuela tiene un lugar destacado en la producción de la demanda de cuidados y la familia aún no está muy involucrada en la construcción de acciones. Se concluye que se necesitan avances para ofertar una atención guiada por lineamientos de la política del SMIJ.

Resumo Este artigo pretende conhecer como a rede de cuidados em saúde tem se operacionalizado a partir da percepção de familiares de crianças com demanda de cuidado em saúde mental (SM). Foram realizados dois grupos focais, um com familiares da Atenção Básica (AB) e outro com familiares do Centro de Atenção Psicossocial Infantojuvenil (CAPSij), totalizando 15 participantes. Seguiu-se com a análise lexical do tipo classificação hierárquica descendente, com o auxílio do software R Interface, a fim de análises multidimensionais de textos e questionários (IRaMuTeQ), resultando em cinco classes: A Pílula Mágica; Forças e Fraquezas dos serviços; Procurando por ajuda; Aceitando o diagnóstico da criança e Onde procurei ajuda. Os resultados apontam para dificuldades presentes na AB em identificar e manejar situações de Saúde Mental Infantojuvenil (SMIJ), por meio de uma lógica ainda medicalizante. Ressalta-se que a escola é apresentada como lugar de destaque na produção da demanda por cuidado e a família ainda é pouco convocada à construção das ações. Conclui-se, então, que avanços ainda são necessários para operacionalização de um cuidado pautado nas diretrizes da política de SMIJ.

Abstract Sarcopenia affects performance in simple activities of daily life, directly impacting the quality of life of the elderly. The objective of the present study was to analyse the correlation between age, sarcopenia index and functional capacity in community-dwelling elderly. The sample comprised 40 community-dwelling elderly, 12 men (69.16± 8.13 years) and 28 women (67.96± 6.23 years). All participants underwent the Electrical Bioimpedance (BIA), Handgrip Strength test (HS), and Timed-Up-and-Go test (TUG). A strong correlation was found between age x TUG in men (r= 0.733; p= 0.021), between age x fat mass in women (r= 0.775; p= 0.032), between HS and TUG in men (r= − 0.713; p= 0.0003), and a weak correlation between HS and Free Fat Mass in women (r= 0.394; p= 0.043). Weak negative correlations were found regarding age in both men and women. In functional performance, concerning test time and age, strong correlations were found for men and weak correlations for women.

Resumo Objetivou-se compreender as concepções de profissionais de saúde sobre a relação de uma gerência com os diversos serviços que compõem a rede de saúde pública de um município da região sudeste do Brasil. Utilizou-se da técnica do Grupo Focal (GF) para a coleta de dados. Foram realizados três GF com a composição média de 12 participantes por encontro, totalizando 38 participantes. Seguiu-se com a análise lexical do tipo Classificação Hierárquica Descendente com o auxílio do software Iramuteq. Os resultados delinearam quatro classes: “Relação Gestão-Serviço”; “Desafios cotidianos dos serviços”; “Sustentação do trabalho no território” e “Priorização do usuário”. O estudo indica que a relação serviços-gestão é marcada por práticas hierarquizadas. Contudo, foi possível verificar que existem expectativas em torno da instauração de uma relação transversalizada. Pondera-se os espaços coletivos e a participação dos usuários na gestão do cuidado como elementos importantes para a cogestão.

Abstract The objective of this work was to understand the conceptions of health professionals about the relationship between management and the different services that constitute the public health network of a municipality in the southeastern region of Brazil. It used the Focus Group (FG) technique for data collection. Three FG were held with an average of 12 participants per meeting, totaling 38 participants. The Lexical Descending Hierarchical Classification analysis was applied with the support of the Iramuteq software. The results outlined four classes: Management-Service Relationship; Daily challenges of services; Sustainability of work in the territory and Prioritization of the user. The relationship between health services and management was marked by hierarchical practices. However, it was possible to verify that there are expectations regarding the establishment of a transversal relationship. Collective spaces and user participation in care management are considered as important elements for co-management.

Resumen El objetivo de este trabajo fue comprender las concepciones de los profesionales de la salud sobre la relación entre la gestión y los diferentes servicios que componen la red pública de salud de un municipio de la región sudeste de Brasil. Se utilizó la técnica de Grupo Focal (GF) para la recolección de datos. Se realizaron tres GF con un promedio de 12 participantes por reunión, totalizando 38 participantes. Un análisis léxico de Clasificación Jerárquica Descendente fue aplicado con el apoyo del software Iramuteq. Los resultados distinguieron cuatro clases: “Relación Gestión-Servicio”; “Retos cotidianos del servicio”; “Sostenibilidad del trabajo en el territorio” y “Priorización de usuario”. El estudio indicó que la relación entre los servicios de salud y la gestión está marcada por prácticas jerárquicas. Sin embargo, se pudo constatar que existen expectativas en cuanto al establecimiento de una relación transversal. Los espacios colectivos y la participación de los usuarios en la gestión de la atención se consideran elementos importantes para la cogestión.

ABSTRACT: Common bean is a worldwide important crop. The development of varieties with durable resistance to diseases is a major challenge in common bean breeding. The present study aimed at evaluating the phenotypic and molecular selection of anthracnose resistance in a population obtained by assisted backcrossing from IAC Formoso (resistant, donor parent) × BRS Pérola (susceptible, recurrent parent). Nine microsatellites (SSRs) and one Sequence Tagged Sites (STS) markers previously linked to ANT resistance were used to genotype this progeny, and the results showed that the selection of the genotypes closest to the donor parent in the BC1F1 population decreased the number of backcrossing cycles necessary to obtain advanced isogenic lines, potentiating the use of this tool for early selection of resistant cultivars. A total of 31 % of the BC1F1 progeny was selected and backcrossed again. The progeny derived from the second backcross (BC2F3) was selected for the Carioca grain ideotype, and 42 % of the genotypes showed high resistance to anthracnose under controlled conditions of infection for races 65 and 81. Superior resistant plants were selected and evaluated under natural conditions of infection to fusarium wilt and angular leaf spot, allowing the selection of two inbred lines with higher resistance to anthracnose, fusarium wilt, angular leaf spot and postharvest quality traits such as yield, 100 seed weight, L value at seed harvest grain darkening and cooking time. The approach outlined in this paper proved to be effective to simultaneously select for disease resistance without losing technological quality aspects of the bean.

Resumo O Sistema Único de Saúde se sustenta pelos pressupostos do trabalho coletivo, de relações transversalizadas e do compartilhamento de saberes e práticas. Tais princípios, todavia, encontram dificuldade de efetivação perante o modelo biomédico e o desmonte da saúde pública nos últimos anos. Assim, objetivou-se analisar as relações entre profissionais de uma equipe gestora municipal e profissionais dos serviços de saúde. Para isso, realizou-se sete grupos focais com profissionais de gerência e trabalhadores e diretores de serviços de saúde, totalizando 100 participantes. As transcrições dos grupos compuseram um corpus que foi submetido à análise lexical do tipo classificação hierárquica descendente com o auxílio do software Iramuteq, originando cinco classes: “Cotidiano dos Serviços”, “Metáforas da Relação Gestão-Serviço”, “Cotidiano da Gerência”, “Construir Junto?” e “Relação Gestão e Serviço”. A relação gestão-serviços é marcada por ambiguidades. Ora é percebida como distante, e os profissionais da gestão são tidos como os que planejam e decidem e os dos serviços, como os que executam. Ora é vista como lugar de construção conjunta de projetos coletivos em torno de um sistema que dá certo. Isso afirma a discussão de um hibridismo entre o paradigma dominante e o paradigma emergente. Esse último com a intenção de incluir no rol das práticas cotidianas da saúde um olhar para as subjetividades presentes e a perspectiva da construção de relações transversalizadas entre os diversos atores sociais, demandando espaços que potencializam coletivos capazes de promover cogestão.

Abstract The Unified Health System is grounded on the assumptions of collective work, cross-sectional relationships, and the sharing of knowledge and practices. However, these principles find are difficult to implement in the face of the biomedical model and the dismantling of public health in recent years. Therefore, this work aimed to analyze the relationships between professionals from a municipal management team and health service professionals. Hence, seven focus groups were held with management professionals and workers and directors of health services, a total of 100 subjects. The groups’ transcriptions composed a corpus that was subjected to lexical analysis of the descending hierarchical classification type with the support of Iramuteq software creating five classes: “Service Day-to-Day,” “Management-Service Relationship Metaphors,” “Management Day-to-Day,” “Building Together?,” and “Management and Service Relationship.” The management-service relationship is marked by ambiguities. Sometimes it is perceived as distant, and the management professionals considered as those who plan and decide and the services professionals as those who perform. Other times it is seen as a place of joint construction of collective projects around a system that works. This affirms the discussion of a hybridism between the dominant paradigm and the emerging paradigm. The latter with the intention of including in the list of daily health practices a look at the subjectivities present and the perspective of building cross-sectional relationships among the various social actors, demanding spaces that potentiate collectives capable of promoting co-management.

Resumen El Sistema Único de Salud es sustentado por las premisas del trabajo colectivo, por las relaciones transversales y por compartir saberes y prácticas. Sin embargo, tales principios encuentran dificultades frente al modelo biomédico y al desmantelamiento de la salud pública en los últimos años. Por esta razón, se buscó analizar las relaciones entre los profesionales de un equipo gestor municipal y los profesionales del servicio de salud. Para esto, se determinó siete Grupos de Enfoque con profesionales de una gerencia, trabajadores y directores del servicio de salud, con un total de 100 personas. Las transcripciones de los grupos constituyeron un cuerpo que fue sometido a un análisis léxico del tipo Clasificación Jerárquica Descendiente, con el auxilio del software Iramuteq, originando cinco clases: “Cotidiano de los servicios”, “Metáforas de la relación gestión-servicio”, “Cotidiano de la Gerencia”, “¿Construir junto?” y Relación Gestión y Servicio. La relación gestión-servicio está marcada por ambigüedades. Puede ser percibida como distante, y a los profesionales de gestión como los que planean y deciden, y a los de servicio, como los que ejecutan. También puede ser vista como un lugar de construcción conjunta de proyectos colectivos en torno a un sistema que funciona de manera correcta. Eso afirma la discusión de un hibridismo existente entre el paradigma dominante y el paradigma emergente. Este último con la intención de influir en el rol de las prácticas cotidianas de la salud una aproximación a las subjetividades presentes y la perspectiva de construcción de relaciones transversalizadas entre los diversos actores sociales, demandando espacios que potencialicen a colectivos, capaces de promover la cogestión.

Abstract Common bacterial blight (CBB) is an important disease in Phaseolus vulgaris L. A carioca diversity panel (CDP) composed of 149 cultivars of common bean was genotyped with 1,616 SNPs and evaluated for Xanthomonas phaseoli pv. phaseoli (Xap) resistance aiming to identify QTLs. Phenotypic evaluation was done in controlled conditions. The plants displayed in a randomized block design, with 3 replications were evaluated ten days after inoculation. GWAS analysis using the BLINK model identified one SNP on chromosome Pv07, with a different genomic location in relation to previous studies. Considering a confidence interval of 100 kb, gene annotation identified 13 candidate genes related to defense-associated genes, and their key functional motives were discussed. The marker identified may constitute an important resource for future marker assisted CBB resistance breeding.

RESUMO JUSTIFICATIVA E OBJETIVOS: Estudos demonstraram que o receptor canabinóide CB1 está envolvido na modulação da dor, principalmente pela ativação da via descendente de controle da dor, porém o papel da fotobiomodulação nesse processo não é bem elucidado. Assim, o presente estudo teve como objetivo investigar o envolvimento do receptor CB1 na antinocicepção induzida pela fotobiomodulação a nível supraespinhal. MÉTODOS: Camundongos machos suíço albinos foram submetidos à lesão por constrição crônica e tratados com fotobiomodulação. Para avaliar o envolvimento supraespinhal do receptor CB1 na antinocicepção induzida por fotobiomodulação foi injetado o antagonista do receptor canabinóide CB1, AM251 (0,1µg/vol 0,2µL) 5 minutos antes do tratamento com fotobiomodulação. O tratamento de fotobiomodulação foi realizado no quinto dia após cirurgia estereotática e lesão por constrição crônica, na dose de 50J/cm2 em estado agudo. Os testes de placa quente e monofilamentos de von Frey foram realizados para avaliar a sensibilidade térmica e mecânica à dor, respectivamente. RESULTADOS: O limiar térmico e mecânico nociceptivo foi maior nos camundongos com lesão por constrição crônica, injetados com solução salina e tratados com fotobiomodulação na dose de 50J/cm2 nos testes de placa quente (p<0,001) e von Frey (p>0,001). Esses efeitos antinociceptivos não foram detectados em camundongos com lesão por constrição crônica tratados com AM251. CONCLUSÃO: O presente estudo sugere que os receptores CB1 localizados nas estruturas supraespinhais participam do controle da dor neuropática, após tratamento com fotobiomodulação em animais submetidos à lesão por constrição crônica.

ABSTRACT BACKGROUND AND OBJECTIVES: Studies have demonstrated that the cannabinoid CB1 receptor is involved in the modulation of pain, mainly by activating the descending pain control pathway. However, the role of photobiomodulation in this process is not well elucidated. Thus, the present study aimed to investigate the involvement of the CB1 receptor in the supraspinal photobiomodulation-induced antinociception. METHODS: Male albino swiss mice were submitted to chronic constriction injury and treated with photobiomodulation. To evaluate the supraspinal involvement of the CB1 receptor in the photobiomodulation-induced antinociception, the cannabinoid CB1 receptor antagonist AM251 (0.1µg/vol 0.2µL) was injected 5 minutes before the photobiomodulation treatment. The photobiomodulation treatment was performed on the fifth day after the stereotactic surgery and chronic constriction injury at a dose of 50J/cm2 in acute condition. The hot plate and von Frey monofilaments tests were performed to evaluate the thermal and mechanical pain sensitivity, respectively. RESULTS: The thermal and mechanical nociceptive threshold was higher in mice with chronic constriction injury, injected with saline and treated with photobiomodulation at the dose of 50J/cm2 in both the hot plate (p<0.001) and von Frey (p>0.001) tests. These antinociceptive effects were not detected in mice with chronic constriction injury pre-treated with AM251. CONCLUSION: The present study suggests that CB1 receptors located in Supraspinal structures, participate in the control of neuropathic pain following photobiomodulation treatment in animals undergoing chronic constriction injury.

Abstract IAC Nuance and IAC Tigre are special common bean cultivars for consumption in Brazil and for international markets. IAC Nuance has a 75-day cycle, with cranberry type rajado (streaked/dappled) bean seeds. IAC Tigre has 85-day cycle, a cream-colored seed coat with brown specks (pinto bean type). These cultivars are moderately resistant to anthracnose, angular leaf spot, fusarium wilt, common bacterial blight, and bacterial wilt.

Abstract This study aimed to evaluate the resistance of three common bean genotypes (BRS Estilo, A211 and Mortiño) to the Fusarium oxysporum f. sp. phaseoli using different inoculation methods such as the root-dip, the colonized toothpick, and the agar-based disk-diffusion. After 35 days of inoculation, the disease severity was assessed by vascular discoloration on the stem of the plants by two methods: using a scoring scale and measuring the length of the discoloration with the aid of a millimeter ruler (cm). Results showed that the root-dip was the most effective inoculation method. As for the method of assessing the disease severity, the scoring scale was the best one, in addition to being easier for evaluating large amounts of common bean lines.

Abstract IAC 1849 Polaco is a common bean cultivar with a carioca (beige/cream-colored with brown stripes) seed coat, 75-day mean maturity , semi-upright plant architecture, tolerance to seed darkening, mean 1000-seed weight of 240 grams, resistance to the main diseases in common bean, and mean seed yield of 2464 kg ha-1 obtained in 18 experiments.

Abstract IAC Netuno, a black common bean cultivar with high grain yield potential, 2968.20 kg ha-¹, upright plant architecture and Type II indeterminate growth habit, about a 90-day cycle, and 1000-seed weight of 230 grams, recommended for all common bean crop seasons in the state of São Paulo.

Abstract The black bean cultivar IAC Veloz developed by the dry edible bean (or common bean) breeding program of the Instituto Agronômico-IAC stands out because of its early cycle, upright plant type, 1000 seed weight of 220 grams, and mean yield potential of 3770 kg ha-1 in a total of 18 environments.

Abstract Fusarium oxysporum f. sp. phaseoli (Fop) J.B. Kendrich & W.C. Snyder is the causal agent of Fusarium wilt of common bean (Phaseolus vulgaris L.). The objective of this study was to develop microsatellite markers (SSRs) to characterize the genetic diversity of Fop. Two libraries enriched with SSRs were developed and a total of 40 pairs of SSRs were characterized. Out of these, 15 SSRs were polymorphic for 42 Fop isolates. The number of alleles varied from two to ten, with an average of four alleles per locus and an average PIC (Polymorphic Information Content) of 0.38. The genetic diversity assessed by microsatellites for Fop was low, as expected for an asexual fungus, and not associated with geographic origin, but they were able to detect enough genetic variability among isolates in order to differentiate them. Microsatellites are a robust tool widely used for genetic fingerprinting and population structure analyses. SSRs for Fop may be an efficient tool for a better understanding of the ecology, epidemiology and evolution of this pathogen.